Abstract: Two methods for isolating HP-7 (Ustilaginoidea virens) and 7 fungicides for controlling the false smut disease on rice caused by the pathogen were compared. Fungal spores at different maturation stages were separated by means of shaking or flame burning. HP-7 was verified by its morphology, pathogenicity, and rDNA-ITS sequences. The sensitivity of HP-7 toward and toxicity on the fungi by the fungicides were determined based on the fungal mycelium growth under treatment. It was found that the successful rate in separating the fresh yellow chiamydospores by shaking was up to 98.33%, which was better than the flaming method. The rDNA-ITS sequences of HP-7 were submitted to NCBI for a BLAST analysis that showed a 99% homogeny with U. virens. Combining that result with the morphological observations, HP-7 was verified to be the pathogen that caused the rice false smut. Among the fungicides tested, tebuconazole and azoxystrobin exhibited the highest inhibition rates on HP-7, with EC₅₀ at 0.109 1 and 0.158 5 mg·L^-1, respectively. It appeared that an effective spore separation method was identified, and the positive identification and appropriate selection on fungicide for effective control of the false smut disease on rice crops were obtained.