Abstract: Fusarium wilt (F.W) disease-resistant molecular markers can be used to screen disease-resistant lines in early planting stage of sweet potato, which can also greatly improve the efficiency of breeding for sweet potato disease-resistance.The F1 progenies were obtained from the cross of sweet potato c.v. Jinshan57, which is higly resistant to Fusarium wilt (F.W), and c.v. Xinzhonghua, which is highly susceptible to F.W. After disease-resistant identification, 7 highly disease-resistant and 7 highly disease-susceptible lines were selected from F1 progenies and were used to establish the resistant and susceptible DNA pools. The genomic DNA of the resistant and susceptible pools were PCR-amplified respectively with 276 pairs of SRAP primers, and 98 pairs of primer generated polymorphic bands between the disease-resistant and susceptible pools. Through further screening using a group of small population and their parents, we got a SRAP marker named a12b8, which would be linked to the resistance of F.W. in sweet potato. This marker would be used for identifying the resistance to F.W in the early growth stage of sweet potato and also would be utilized for improving the efficiency of F.W disease-resistant breeding by molecular marker assisted selection for sweet potato.