辣椒EST-SSRs标记的通用性研究

Cross-species Applicability of EST-SSRs from Capsicum annuum L.

摘要: 为研究辣椒EST-SSRs标记在主要茄科蔬菜间的通用性，从NCBI数据库下载118 900条辣椒相关EST，对其进行SSR位点搜索，用含SSR位点的辣椒ESTs设计的200对EST-SSRs引物对17份辣椒材料、24份番茄材料、16份茄子材料进行PCR扩增，有效扩增比率分别为98%、94.5%、90.5%，多态性比率分别为35.5%、29.5%、26.5%。多态性引物对辣椒、番茄、茄子的特定亲本及其杂种一代F1的亲缘关系进行检测，均能达到预期效果，试验结果说明从辣椒相关EST数据库中开发的SSR引物在茄科植物中有较好的通用性。

Abstract: The applicability of the EST-SSRs marker identified on Capsicum annuum L for uses on other solanaceae vegetables was investigated. A total of 118, 900 ESTs in C. annuum L were downloaded from NCBI. Two hundred pairs of EST-SSR primers were developed. Approximately 98%, 94.5% and 90.5% of them were successfully amplified in C. annuum L., Solanum lycopersicum and S. melongena L., respectively, with the polymorphism proportions of 35.5%, 29.5% and 26.5%, respectively. The polymorphic primers specific to the parents and their F1 of C. annuum L., S. lycopersicum and S. melongena L. were tested with the anticipated results. It appeared that the SSR primers developed from the EST database related to C. annuum L. could be applied on solanaceae plants with satisfaction.