Abstract: To select desirable parents for cross-pollination, effect of severalvarieties of Fuguihong pitaya (Hylocereus undulatus Britt) on the properties of the fruits was studied. The appearance, eating quality, fruiting, cracking and development time of the fruits, as well as characteristics of the seeds produced from the flowerscross-pollinated between Fuguihong and 6 varieties of pitaya were compared.Significant improvements on fruiting percentage, single fruit weight, seed count and weight per thousand grains, as well asreductions onincidents of fruit cracking were found because of the cross-pollination. Among the pollen-donor plants, Mihong, Jinduyihao and Dahong also provided the resulting fruits with large edible portion, high soluble solids, and attractive flesh color and flavor; while, Mihong, Jinduyihao, Dahong and Xixianghong, increased totalsugars, vitamin C, and total amino acids, butreduced total acid.Therefore, it appeared that using Mihong, Jinduyihao and/or Dahong pitaya to pollinate the flowers on Fuguihong could enhancethe fruit qualities.