Abstract: The transition from dormancy to germination in seeds is a key physiological process during the lifecycle of plants. Hormones play an important regulatory role on it, While, short-chain dehydrogenase/reductase (SDR1) is a molecular link between nutrient signaling and plant hormone biosynthesis. The coding sequences (CDS) of SDR1 were cloned from different dormancy varieties FH7185, FH653 and YH290, respectively. The results of sequences blast indicated that the coding regions of OsSDR1 among the three varieties were homologous, but there were 9 bases deletion, coding three alanine compared to Nipponbare. Meanwhile, the results of real-time quantitative PCR (qRT-PCR)of SDR1 expression level in seed embryo among different dormancy varieties indicated that the expression level of SDR1 was significantly different among them. Moreover, the corresponding interaction proteins of OsSDR1 were screened by GST pull-down experiments and an interaction protein of OsSDR1 was found and its Genbank serial number designated as Os05g0140800, it was verified related to seed dormancy. The preliminary results showed that SDR1 gene might regulate seed dormancy through interaction with other structural elements.