Abstract: The protocorm-like body(PLB) induced from stem tips of Dendrobium spp. Sanya Sunny' were used as explants, The orthogonal design and the liquid media were used to study the effects of the key factors such as culture medium, TDZ, coconut water and sugar contained on PLB propagation of Dendrobium spp. 'Sanya Sunny'.Through above research, it was tried to find the main factors and build up the PLB proliferation the system in Dendrobium spp. Sanya Sunny' in the paper. The results showed that the main factors of PLB propagation are the basic medium, TDZ, sugar and coconut water. The best multiplication medium of PLB is improvement HM#2 medium, TDZ 0.5 mg·L^-1, NAA 0.1 mg·L^-1, coconut water 20.0 mL·L^-1, and sugar 30.0 g L^-1 to result in an averaged propagation coefficient of 6.25 in 35 days.