产N-羟基异戊巴比妥的芽胞杆菌筛选

N-hydroxyamobarbital-producing Bacillus-like Strains

摘要: 采用液相-四级杆飞行时间质谱的方法筛选产N-羟基异戊巴比妥（N-Hydroxy Amobarbital）的芽胞杆菌菌株。通过分析73株芽胞杆菌菌株发酵液中胞外化合物成分，筛选出合成N-Hydroxy Amobarbital的4株芽胞杆菌，为栗褐芽胞杆菌Bacillus plakortidis（FJAT-8757）、内生芽胞杆菌Bacillus endophyticus（FJAT-10010）、米氏解硫胺素芽胞杆菌Aneurinibacillus migulanus（FJAT-14205）和休闲地芽胞杆菌Bacillus novalis（FJAT-14227），该成分在发酵液中的相对含量分别为6.04%、4.85%、1.61%和1.61%，ATPA与色谱库检索得分（Score）分别为96.25、94.33、96.72和94.91。

Abstract: Bacillus-like bacteria were screened for their N-hydroxyamobarbital productivities. Extracellular metabolites of 73 candidates were analyzed using the liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry (LC-QTOF MS) and Metlin database. The relative contents of N-hydroxyamobarbital in the culture media on strains that showed substantial levels were 6.04%, 4.85%, 1.61% and 1.61% for B. plakortidis FJAT-8757, B. endophyticus FJAT-10010, Aneurinibacillus migulanus FJAT-14205, and B. novalis FJAT-14227, respectively. The specialized m/z scores on FJAT-8757, FJAT-10010, FJAT-14205 and FJAT-14227 matched to ATPA were 96.25, 94.33, 96.72 and 94.91, respectively.