Abstract: Genetic characteristics and variation of the epidemic strain of duck Tembusu virus (DTMUV) found in Jiangxi Province were investigated. A DTMUV strain, designated as YF, was isolated from a clinical case in 2013. A sequence analysis showed the viral genome of YF to be 10, 990 nt in length with no Ins/Del or genetic recombination. It shared no less than 98.5% homology with the viral strains isolated from ducks since 2010. Phylogenetically, YF had a close relationship with currently circulating dominant virus lineage. It appeared that the DTMUV in local ducks had been genetically stable throughout these years.