Abstract: Genetic relationships among 196 grape cultivars collected from various regions in Fujian were determined using ISSR-PCR. The 12 selected primers amplified 164 bands from the plants with up to 91.44% polymorphic loci. The clustering analysis revealed that the germplasms could be divided into three categories as the eastern Asia population, the inter-specific hybrids (Vitis vinifera V. labrusca) and V. vinifera. A population genetic polymorphism analysis on the cultivars showed abundant diversities among them.