Abstract: Antigenicity difference among strains of pseudorabies virus (PRV) and potential of developing a vaccine to prevent new PRV from occurring were studied. Four PRV strains (i.e., PRV FB, FA, Bartha-K61 and prevalent PRV-FJ2012) and hyperimmune serums were used for the analysis by a cross neutralization test. The possibility of using PRV-FB to develop a vaccine was evaluated by a challenge test. The neutralization titers of hyperimmune serum for rabbit anti-FB, anti-FA, anti-Bartha-K61 and anti-FJ2012 were 1:54, 1:91, 1:91, and 1:215, respectively. The correlation coefficients between FB and FA, FB and FJ2012, and FA and FJ2012 were all greater than 0.8. However, those between Bartha-K61 and FA, Bartha-K61 and FB, and Bartha-K61 and FJ2012 were lower than 0.4. The results indicated that the relationship between the antigens of FB and FJ2012 was closer than that of Bartha-K61 and FJ2012. The viral challenge test showed that FB could prevent PRV-FJ2012 at a rate of 100%.These conclusion indicated FB strain was a vaccine candidate strain to prevent the new PRV strain(FJ2012 strain).