• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

Ca(NO3)2对百香果插穗生根及抗氧化酶活性的影响

Effects of Ca(NO3)2 on Rooting and Leaf Antioxidase Activities of Cuttings for Propagating Passiflora edulis×P. edulis f. flavicarpa

    摘要
  • 摘要: 以百香果枝条作插穗，采用不同质量浓度的Ca（NO3）2处理，研究Ca（NO3）2对插穗生根及其叶片过氧化物酶（POD）、超氧化物歧化酶（SOD）、过氧化氢化酶（CAT）、抗坏血酸过氧化物酶（APX）活性和膜质过氧化物产物丙二醛（MDA）含量的影响。试验结果表明，当质量浓度为50 mg·L-1 Ca（NO3）2处理的生根数测得值为26.8条，根鲜样质量测得值为0.9 g·plant-1，根系活力测得值为220.56 μg·h-1·g-1FW，与对照相比达到差异显著；质量浓度为50 mg·L-1 Ca（NO3）2处理的POD、SOD、CAT和APX活性测得值均比其他处理显著增加，且MDA含量测得值均比其他处理显著减少。因此，适当质量浓度的Ca（NO3）2处理可提高POD、SOD、CAT和APX活性，降低MDA含量，从而提高插穗对逆境的抵抗及愈合能力，促进插穗生根。

     

    Abstract: Effects of Ca(NO3)2 in varied concentrations on the rooting and activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and malondialdehyde (MDA) in leaves of Passiflora edulis P. edulis f. flavicarpa cuttings for the plant propagation were studied. The results showed that the application of 50 mg Ca(NO3)2/L significantly increased the count, fresh mass, and vitality of the roots per plant as compared to control. The activities of POD, SOD, CAT and APX in the leaves were also enhanced significantly, while MDA significantly reduced. It suggested that Ca(NO3)2 treatment at a proper dosage could promote rooting of the cuttings as a result from the increased antioxidant enzyme activities and decreased membrane lipid peroxidation in the leaves of the seedlings.

     

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