台湾速生杨树离体再生体系的建立

Tissue Culture Regeneration of Fast-growing Taiwan Poplar

摘要: 以台杨3号茎段和叶片为外植体材料，建立离体再生体系。结果表明：茎段在0.15% HgCl₂溶液中处理10 min，污染率为23.3%，出芽率达75.3%，在MS+BA 0.3 mg·L^-1+NAA 0.1 mg·L^-1培养基中诱导不定芽的效果较好，平均分化率77.8%；叶片分化培养基为MS+BA 0.5 mg·L^-1+NAA 0.2 mg·L^-1+KT 0.2 mg·L^-1+Ad 40 mg·L^-1，分化率可达87.7%；MS+BA 0.5 mg·L^-1+KT 0.3 mg·L^-1+NAA 0.2 mg·L^-1培养基增殖效果较好；1/2MS+NAA 0.2 mg·L^-1+CCC 0.5 mg·L^-1为生根、壮苗培养基，生根率100%。

Abstract: A regeneration protocol was established using the stems and leaves of fast-growing Taiwan Poplar 3 as explants. The selected disinfection time for the stems was 10 min in a 0.15% HgCl₂ solution to result in an infection rate of 23.3% and a budding rate of 75.3%. The optimized medium for generating adventitious buds was MS+BA 0.3 mg·L^-1+NAA 0.1 mg·L^-1 to reach an average differentiation rate of 77.88%; that for the leaf differentiation, MS+BA 0.5 mg·L^-1+NAA 0.2 mg·L^-1+KT 0.2 mg·L^-1+adenine 40 mg·L^-1 with a differentiation rate of 87.7%; that for the proliferation, MS+BA 0.5 mg·L^-1+KT 0.3 mg·L^-1+NAA 0.2 mg·L^-1; and that for rooting, 1/2MS+NAA 0.2 mg·L^-1+CCC 0.5 mg·L^-1 with a 100% rooting rate.