Abstract: SRAP molecular markers were used to access the genetic diversity of 48 Dendrobium germplasms. Using 14 pairs of selected primers, 159 bands were amplified. Of the bands, 155 were polymorphic. The polymorphism rate was 90%-100% per primer, and the information content in the markers ranged abundantly from 0.718 to 0.903. Cluster analysis showed the genetic distances among the 48 cultivars to be 0.15-0.97. At the distance of 0.83, the germplasms could be classified into 7 groups. The results showed a rich genetic diversity among the germplasms.