Abstract: The rich content of anthocyanidin in purple Abelmoschus esculentus renders the characteristic color of the plant and its fruits. This study used the transcriptome of the plant to study the genetics associated with the anthocyanidin biosynthesis pathway under natural conditions. The transcriptome was sequenced using the Illumina HiSeq 2500 platform to isolate 35 anthocyanidin unigenes from the Nr, SwissProt and GO databases. These unigenes belonged to 4 distinct gene types including 19 of anthocyanidin-related glucosyltransferases, 6 of anthocyanidin-related acyltransferases, 5 of leucoanthocyanidin dioxygenases, and 5 of anthocyanidin-related reductases. The genes were annotated by Nr and SwissProt databases to show their associations with a variety of plants, such as Arabidopsis thaliana, Theobroma cacao, Gossypium arboretum, Manihot esculenta, Malus domestica and Vitis vinifera. A clustering analysis indicated that the unigenes had the closest relationship with the leucoanthocyanidin dioxygenase family, followed by the anthocyanin-related acyltransferases and the reductases, while the anthocyanidin-related glucosyltransferases showed the farthest homology. Identification of the 35 anthocyanidin-related unigenes would be useful for the gene cloning and analysis in studying anthocyanin biosynthesis of purple A. esculentus or other plants rich in anthocyanidin.