Abstract: Lifespan of Caenorhabditis elegans in vivo, along with the DPPH radical scavenging test in vitro, was used to evaluate the anti-oxidative activity of Chlorophyllum molybdites. The results showed that the C. molybdites fermentation broth increased the resistance of the nematodes to oxidative stress. At 0.5 mg·mL^-1, the broth extended the mean lifespan of the paraquat-treated C. elegans 70% longer as compared to that of CK. The anti-oxidative activity of the water extract of immature fruitbodies was greater than that of the mature fruitbodies of C. molybdites. When treated with the water extract of immature mushrooms at 0.5 and 1.0 mg·mL^-1, the mean lifespans of the nematodes were 4.85 d and 5.06 d, respectively, which were significantly longer than that of CK. The DPPH scavenging rate of the fermentation broth was the highest, at 98.14%, followed by that of the water extract of mature fruitbodies, at 92.86%, and that of the water extract of immature fruitbodies, at 85.71%. The findings suggested that C. molybdites could potentially be a new source for producing antioxidant from a biomaterial.