Abstract: The protocorms and in vitro plantlets of Dendrobium huoshanense were used in this study.Effects of various plant growth regulators on the protocorm proliferation, as well as, those of differently formulated media with additions of potato powder, active carbon and/or IBA on the plantletgrowth and rooting were evaluated. It was found that the in vitro culture, which best enhanced the protocorm proliferation, applied 1/2MS supplemented with 2, 4-D 0.2 mg·L^-1, 6-BA 0.2 mg·L^-1 and KT 0.4 mg·L^-1, with 6-BA being the most important factor among the growth regulators tested. For strong growth with robust rooting of the plantlets, the determining factor was active carbon in a medium containing 1/2MS, potato powder 1.25 g·L^-1, active carbon 0.5 g·L^-1, and IBA 0.4 mg·L^-1.