• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

甘薯曲叶病毒福建省分离物的分子鉴定

Molecular Identification for Sweet Potato Leaf Curl Virus Isolated in Fujian

  • 摘要: 为了鉴定福建省甘薯曲叶病毒Sweet potato leaf curl virus,SPLCV,并分析其病原的分子特征,根据已报道的SPLCV基因组序列设计特异性引物,对获得的特异性片段进行回收、克隆、测序。根据测序结果设计了扩增SPLCV基因组全长引物(SPLCVQ),同时利用已报道的DNA-B的通用引物PCRc1/PBLlv进行扩增,克隆获得SPLCV分离物基因组全长为2 827 bp,该序列含有6个开放阅读框,只含有DNA-A组分,不含有DNA-B组分(GenBank登录号:KT202303)。核苷酸同源性分析结果表明,该分离物与河南、江苏、浙江、韩国、美国亲缘关系最近,处于同一亚组的不同分支中。

     

    Abstract: Molecular characteristics of the sweet potato leaf curl virus (SPLCV) in Fujian province were studied. Specific segments of the genome were recovered, cloned and sequenced using selected primers. A sequence analysis positively confirmed the segments to be a part of SPLCV. Then, the full-length SPLCV was constructed with a pair of primers Q, and sequenced. The previously reported general purpose primers, PCRc1/PBLlv, for DNA-B were used for the subsequent amplification. The genome of SPLCV was found to be 2 827 bp containing 6 ORFs with DNA-A but not DNA-B (GenBank accession number:KT202303). The nucleotide homology between the isolated genome and the reported 6 other geminiviridae genomes was less than 85%;and, between it and SPLCV was the highest at 95.94%. In addition, the isolate shared a close genetic relationship with other isolates from Henan, Jiangsu, Zhejiang, South Korea, and the United States belonging to a same subgroup but in different branches.

     

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