Abstract:
Molecular characteristics of the sweet potato leaf curl virus (SPLCV) in Fujian province were studied. Specific segments of the genome were recovered, cloned and sequenced using selected primers. A sequence analysis positively confirmed the segments to be a part of SPLCV. Then, the full-length SPLCV was constructed with a pair of primers Q, and sequenced. The previously reported general purpose primers, PCRc1/PBLlv, for DNA-B were used for the subsequent amplification. The genome of SPLCV was found to be 2 827 bp containing 6 ORFs with DNA-A but not DNA-B (GenBank accession number:KT202303). The nucleotide homology between the isolated genome and the reported 6 other geminiviridae genomes was less than 85%;and, between it and SPLCV was the highest at 95.94%. In addition, the isolate shared a close genetic relationship with other isolates from Henan, Jiangsu, Zhejiang, South Korea, and the United States belonging to a same subgroup but in different branches.