芥菜遗传多样性SRAP分析

SRAP Analysis on Genetic Diversity of Brassica juncea

摘要: 以44个芥菜品种为材料，利用SRAP分子标记对其进行遗传多样性研究。从100个不同的引物组合中筛选出了31对多态性较高，条带清晰且稳定的引物，对44个芥菜材料DNA进行PCR扩增。结果表明，这31对引物共产生了286条条带，其中多态性条带173条，多态率达60.49%。平均每对引物扩增条带9.23条。用NTSYS统计软件进行聚类分析，44个芥菜品种的遗传相似系数为0.61~0.89，平均系数为0.75。在相似系数为0.67的水平，44个芥菜品种可分为4大类，与形态学分类结果基本一致。

Abstract: An SRAP marker was applied to analyze the genetic diversity of 44 accessions of Brassica juncea(L.). Thirty-one pairs of SRAP primer combinations, which amplified stable bands with clear and higher polymorphism, were selected by a preliminary screening. A total of 286 DNA bands were amplified from the 44 accessions of B. juncea by PCR amplification.Among them, 60.49% or 173 DNA bands were polymorphic.The average number of DNA bands amplified by each pair of primer combination was 9.23. The clustering analysis by NTSYS statistical software showed that the genetic similarity coefficients were in the range of 0.61-0.89 averaging 0.75. At the coefficient of 0.67, the 44 accessions were divided into 4 groups which were identical with the result obtained by the morphological classification.