Abstract: To optimize the tissue culture and rapid propagation of Benihoppe strawberry, effects of basic medium and plant growth regulators on the induction, proliferation and rooting of the adventitious buds were studied. The results showed that the disinfection of the strawberry stem tips with 75% alcohol for 30 s and 0.1% HgCl₂ for 7 min satisfactorily rendered a survival rate of the tissue up to 90%. The bud induction was affected by the following factors in the order of basic medium > 6-BA > IBA > NAA. The best medium for the highest induction rate of 92%consisted of MS+ 6-BA 2.0 mg·L^-1+NAA 0.3 mg·L^-1+IBA 0.3 mg·L^-1. The effects of the 3 plant growth regulators in the proliferation stage ranked 6-BA > GA₃ > IBA with the optimal medium containing MS+6-BA 0.50 mg·L^-1+IBA 0.08 mg·L^-1+GA₃0.10 mg·L^-1 resulting in a proliferation coefficient of 13.97. For the rooting, the effects were found to be in the order of basic medium > IBA > sucrose, and the optimal medium to be 1/2 MS+sucrose 30 g·L^-1+IBA 0.4 g·L^-1 to yield a rooting index of 35.40. The best basic medium was peat:perlite at 1:1 ratio that provided a seedling survival rate of 93.33%.The plant growth regulator and concentration used in this experiment further improved the induction and proliferation of Benihoppe strawberry, and obtained a good rooting index and seedling survival rate, and further optimized the rapid propagation system of Benihoppe strawberry.