Abstract: An experiment was conducted using young scapes of Haworthia Tsukikage to study the disinfection of explants for tissue culture and propagation. The choice treatment was determined to immerse the scape cuts in 75% ethanol for 30 s followed by 0.1% HgCl₂ for 5-7 min. The orthogonal experiment rendered the optimized medium for the callus induction to be MS+ 6-BA 2.0 mg·L^-1+NAA 0.3 mg·L^-1+KT 1.0 mg·L^-1; that for the callus differentiation, 6-BA 1.0 mg·L^-1+NAA 0.1 mg·L^-1+KT 1.5 mg·L^-1; and that for the rooting, 1/2MS+NAA 0.1 mg·L^-1+ activated carbon 5 g·L^-1. The survival rate of the transplanted seedlings was approximately 85%. A preliminary method of the tissue culture and rapid propagation for H. Tsukikage was thus established. Further refinement for scaleup operation is in order.