寒兰花总黄酮超声提取工艺

Ultrasound Extraction of Flavonoids from Cymbidium kanran Flowers

摘要: 研究超声提取寒兰花总黄酮的工艺，通过单因素试验和L₉（3⁴）正交试验，分析料液比、乙醇体积分数和超声时间对寒兰花总黄酮得率的影响。结果表明，寒兰花总黄酮的最佳提取工艺条件为料液比1：20，乙醇体积分数90%，超声时间10 min，寒兰花总黄酮得率为2.055 4%，该工艺方法重现性好，稳定性高，加样回收率为98.10%~100.20%。

Abstract: Extracting flavonoids in the flowers of Cymbidium kanran assisted with ultrasonic treatment was studied and the processing conditions optimized. Solid:liquid ratio, ethanol concentration and ultrasound application for the process were optimized by the single factor and L₉(3⁴) orthogonal experiments to arrive at the following conditions:1:20 for the solid:liquid ratio, 90% ethanol as the solvent, and 10 min for the ultrasonic treatment. The resulting flavonoid yield was 2.06%. The method seemed to perform with high reproducibility and stability rendering a flavonoid recovery rate of 98.10%-100.20%.