基于无患子科ITS序列探讨龙荔的系统发育

姜帆; 陈秀萍; 郑少泉

doi:10.19303/j.issn.1008-0384.2019.01.006

基于无患子科ITS序列探讨龙荔的系统发育

ITS-based Phylogeny of Dimocarpus confinis

摘要

摘要:
目的利用无患子科间隔转录区序列（ITS）探讨了龙荔的系统发育地位。
方法通过无患子科27个属62个种ITS序列信息序列比对和系统发育分析，探讨龙荔和龙眼、荔枝之间的系统进化关系。
结果基于ITS序列信息可以清楚区分无患子科内不同的种属，其属间ITS序列相似度为（91.0±4.4）%、种间为（96.3±2.8）%。龙荔与龙眼、荔枝之间的ITS序列同源性均为92%，低于龙眼与荔枝间的同源性（93%），但高于和红毛丹的同源性（91%），其ITS序列中有4.5%的碱基位点与龙眼和荔枝均不同，主要表现为T和C互换、A和G互换，共占序列差异位点的56.5%。以韶子属红毛丹为外类群的系统发育树显示，龙荔、荔枝、龙眼之间的进化发育明显，龙荔最早自成分支，其次是荔枝，最后是龙眼。
结论 ITS序列信息分析结果支持龙荔作为独立属的分类可能。

Abstract:
Objective To establish the phylogeny of longli, Dimocarpus confinis, based on its nuclear encoded internal transcribed spacer (ITS) sequences.
Method The ITS sequences of 27 genera and 62 species of Sapindaceae were collected to examine the relationship among longli, longan (Dimocarpus longan) and litchi (Litchi chinensis).
Result The genera in Sapindaceae family could be clearly classified by ITS sequence. The homology among the genera was(91.0±4.4)%, and among the species (96.3±2.8)%. The ITS sequence homology between longli and longan or litchi was 92%, which was lower than that between longan and litchi at 93% but higher than that between longli and rambutan (Nephelium lappaceum L.) at 91%. Four and half percent of the longli ITS sequence differed from those of longan and litchi. The differences were mainly changing between T and C or A and G that accounted for 56.5% of the total. Based on the phylogenetic tree of rambutan as the outgroup, the evolution of longli was shown to be the earliest to branch out, followed by litchi and longan.
Conclusion The result suggested classifying longli as a separate genus in the family.

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