建兰茎腐病原菌尖孢镰刀菌F-02的绿色荧光蛋白基因标记

姚锦爱; 张鸿; 黄鹏; 陈峰; 余德亿

doi:10.19303/j.issn.1008-0384.2019.01.011

建兰茎腐病原菌尖孢镰刀菌F-02的绿色荧光蛋白基因标记

Green Fluorescent Protein Genetic Marker of Fusarium oxysporum F-02 of Stem Rot Disease on Cymbidium ensifolium

摘要

摘要:
目的开展建兰茎腐病原菌尖孢镰刀菌F-02的绿色荧光蛋白标记研究，直观观察尖孢镰刀菌在建兰植株上的侵染为害，明确病原菌的致病过程，为开展建兰茎腐病原菌的相关研究提供良好技术手段。
方法采用农杆菌介导法对建兰茎腐病原菌尖孢镰刀菌F-02进行绿色荧光蛋白（GFP）转化，并检测转化子的遗传稳定性。
结果 GFP基因可被成功导入到尖孢镰刀菌F-02中并获得表达，转化子在蓝色光源激发下，菌丝和分生孢子均能稳定散发绿色荧光；转化子10次继代培养后菌落生长良好，菌丝和分生孢子仍可稳定散发出绿色荧光，对寄主植物的致病力也未发生改变，
结论 GFP基因可在建兰茎腐病原菌尖孢镰刀菌F-02中稳定存在，并对其致病力没有影响。

Abstract:
Objective Green fluorescent protein(GFP) genetic marker of Fusarium oxysporum F-02 of stem rot disease on Cymbidium ensifolium were conducted in this study in order to observe the inflection of F. oxysporum on C. ensifolium plants, confirm the process of the pathogen, and provide powerful technic for further studies on F. oxysporum.
Method GFP was transformed into F.oxysporum strain F-02 mediated by Agrobacterium, and the genetic stability of GFP in transformants was investigated.
Result GFP gene can be successfully introduced into F.oxysporum and stable green fluorescence can be produced in the hypha and spores under the excitation of blue light source.After 10 successive transfers, the progenies grew well with stable green fluorescence and similar pathogenicity as parental transfrormant.
Conclusion GFP gene was inherited stablely in C.ensifolinm and no effect on pathogenicity in F-02 strain.

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