猪源抗菌肽对禽源沙门氏菌和大肠杆菌的抑菌作用

刘小龙; 赖隆永; 谭礼宁; 刘友霖; 徐磊; 吴昌标; 杨守深; 杨慧; 余勋信; 程龙飞; 黄瑜

doi:10.19303/j.issn.1008-0384.2019.05.010

猪源抗菌肽对禽源沙门氏菌和大肠杆菌的抑菌作用

Effect of Porcine Antibacterial Peptide against Avian Pathogenic Bacteria

摘要

摘要:
目的明确猪源抗菌肽对禽源沙门氏菌和大肠杆菌的抑菌作用，为新型抗菌药物的研发和猪源抗菌肽的生产应用提供科学依据。
方法以猪源抗菌肽为材料，采用液体培养基抑制测定法，结合体内外试验，分别测定猪源抗菌肽对禽源沙门氏菌和禽源大肠杆菌的抗菌活力值，研究其体内外抑菌作用。
结果对不同浓度的抗菌肽抗菌活力值的测定结果表明，与0.5、0.2和0.1 mg·mL^-1的猪源抗菌肽相比较，1.0 mg·mL^-1的抗菌肽对含有10⁶ CFU·mL^-1的菌液抑菌效果最好；与阿莫西林、红霉素、四环素等15种抗生素相比较，猪源抗菌肽对禽源沙门氏菌FJS13株和禽源大肠杆菌FJE61株的抑菌圈最大，其抗菌效果最好；抗菌肽在雏鸡体内的抑菌试验表明，攻毒鸡皮下注射猪源抗菌肽有利于提高雏鸡的存活率，在雏鸡体内也具有一定的抑菌作用。
结论猪源抗菌肽可用于抑制禽源沙门氏菌和大肠杆菌。

Abstract:
Objective Activity of porcine antibacterial peptides (PAP) against avian Salmonella and Escherichia coli was determined for drug development.
Method Inhibitive activity of PAP on avian pathogens was studied in vitro on an liquid medium and by an in vivo test.
Results PAP at a concentration of 1.0 mg·mL^-1 showed a higher bacteriostatic effect on the solution contaning 10⁶ CFU·mL^-1 of the pathogens than at 0.5, 0.2 or 0.1 mg·mL^-1. Compared with 15 antibiotics, such as amoxicillin, erythromycin, tetracycline, etc., PAP induced the largest inhibition zone on the growth of avian Salmonella FJS13 and E. coli FJE61. In a challenging test on chicken, the PAP administration improved the survival rate of the subcutaneous injected birds.
Conclusion PAP could be used as a bioagent to combat the infection on chickens by avian Salmonella and E. coli.

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