嘉宝果叶片总多酚提取工艺优化及其体外降糖活性

林宝妹; 邱珊莲; 郑开斌; 张帅; 洪佳敏; 张少平

doi:10.19303/j.issn.1008-0384.2019.05.012

嘉宝果叶片总多酚提取工艺优化及其体外降糖活性

Process Optimization and in vitro Hypoglycemic Effect of Polyphenols Extracted from Jaboticaba Leaves

摘要

摘要:
目的优化嘉宝果叶片总多酚的提取工艺，检测优化提取后的嘉宝果叶片提取物对α-葡萄糖苷酶的抑制作用。
方法利用超声波辅助与乙醇提取相结合的方法，以总多酚提取量为考察指标，采用单因素及正交试验，研究功率、时间、乙醇体积分数、料液比和温度对嘉宝果叶片总多酚提取量的影响，并采用酶动力学及紫外可见吸收光谱法探讨叶片提取物体外抑制α-葡萄糖苷酶的活性。
结果嘉宝果叶片总多酚的最佳提取工艺为：功率120 W，时间10 min，乙醇体积分数40%，料液比1:160（g:mL），温度40℃，在此条件下，嘉宝果叶片总多酚质量分数可达33.84%。优化提取后的嘉宝果叶片提取物对α-葡萄糖苷酶具有较强的抑制作用，半数抑制浓度IC₅₀为0.003 1 mg·mL^-1，阿卡波糖对α-葡萄糖苷酶的半数抑制浓度IC₅₀为8.110 8 mg·mL^-1；Lineweaver-Burk双倒数作图显示嘉宝果叶片多酚对α-葡萄糖苷酶的抑制类型为反竞争性抑制。
结论正交实验法优化嘉宝果叶片总多酚提取具有可行性，嘉宝果叶片总多酚对α-葡萄糖苷酶具有较强的抑制作用，可作为降血糖功能因子进行开发利用。

Abstract:
Objective To optimize the polyphenol extraction from leaves of jaboticaba and determine the α-glucosidase inhibition by the extract.
Method The ethanol extraction with ultrasound application was optimized by an orthogonal experiment. Effects of the power and time of ultrasonic application as well as the ethanol volume fraction, material-solvent ratio, and processing temperature on the total polyphenols extracted from the leaves were analyzed. The inhibition on α-glucosidase activity by the extract was determined in vitro by an enzyme kinetic study and uv-vis absorption spectrometry.
Result The optimal extraction conditions included the use of 120 W ultrasonic wave for 10 min, 40% ethanol volume fraction, 1:160(g:mL) material-solvent ratio, and 40℃ processing temperature to achieve a polyphenol extraction rate of 33.84%. The α-glucosidase activity was significantly inhibited by the extract with a low IC₅₀ of 0.0031 mg·mL^-1, as compared to IC₅₀ of 8.110 8 mg·mL^-1 on acarbose. The kinetic study by the Lineweaver-Burk plot showed that the polyphenol extract was an anti-competitive inhibitor of α-glucosidase.
Conclusion The polyphenol extract from jaboticaba leaves significantly inhibited α-glucosidase and could be explored for use as a hypoglycemic agent.

HTML全文

参考文献(20)

施引文献

资源附件(0)