解淀粉芽胞杆菌FJAT-8754产纤维素酶和淀粉酶发酵动力学模型的构建

葛慈斌; 蓝江林; 刘波; 王凯; 郑雪芳

doi:10.19303/j.issn.1008-0384.2019.06.011

解淀粉芽胞杆菌FJAT-8754产纤维素酶和淀粉酶发酵动力学模型的构建

Kinetics of Cellulase and Amylase-producing Fermentation of Bacillus amyloliquefaciens FJAT-8754

摘要

摘要:
目的解淀粉芽胞杆菌菌株FJAT-8754是一株高产纤维素酶、淀粉酶的芽胞杆菌，具有在复合饲料酶制剂生产中的应用潜力。为深入了解该菌株的发酵进程，以便实现对发酵过程进行最优化控制。
方法采用50 L发酵罐发酵菌株FJAT-8754，分析发酵过程中菌体数量、pH值、底物浓度、纤维素酶和淀粉酶等的动态变化，并选用Logistic方程和Luedeking-Piret方程构建发酵动力学模型。
结果根据菌株FJAT-8754发酵过程中各参数变化动态建立了模拟菌体生长、纤维素酶与淀粉酶产生和底物消耗的动力学模型，并应用SPSS 16.0软件对数据进行计算与分析，Origin 9.0软件经非线性拟合与优化，获得了最佳模型参数值。
结论所构建的动力学模型的计算值与实验值拟合良好，较好地反映了菌株FJAT-8754发酵过程中菌体生长、底物消耗和酶合成的动力学特征。菌株FJAT-8754发酵过程中产物的合成属于生长部分偶联型。

Abstract:
Objective To construct a kinetic model for the optimized fermentation of Bacillus amyloliquefaciens FJAT-8754, which was capable of effectively producing cellulase and amylase.
Method A 50 L fermenter experiment was conducted to study the cellular growth, pH value, enzyme production and carbohydrate consumption of the fermentation and for subsequent process optimization. A kinetic model was established using the logistic and Luedeking-Piret equations, and experimental data calculated and analyzed with SPSS 16.0 software.
Result The optimal fermentation conditions fitted well in the non-linear model as shown by Origin 9.0 software.
Conclusion The kinetic model obtained accurately reflected the performance of the fermentation. The cellulase and amylase were products of FJAT-8754 metabolism in the culture.

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