饲料中添加裂殖壶菌对斑马鱼抗创伤弧菌感染的影响

陈华; 张丽娟; 李素一; 柯翎; 陈叙; 林晨韬

doi:10.19303/j.issn.1008-0384.2019.10.001

饲料中添加裂殖壶菌对斑马鱼抗创伤弧菌感染的影响

Effects of Feeding Schizochytrium on Anti-Vibrio vulnificus Infection in Zebrafish

摘要

摘要:
目的研究饲料中添加裂殖壶菌对鱼类抗细菌感染能力的影响，为裂殖壶菌作为饲料添加剂的应用提供理论基础。
方法以6个月的成年斑马鱼为研究对象，以基础颗粒饲料为对照组（C组），试验组在基础饲料中添加干重3%的裂殖壶菌（S组），进行28 d饲喂试验。28 d后以创伤弧菌Vibrio vulnificus菌株FJ03-X2对斑马鱼进行攻毒，统计免疫保护率，并收集斑马鱼的肾脏组织提取RNA，逆转录成cDNA，通过Real-time RT-PCR检测相关基因lysozyme和tnfb的表达。
结果饲料中添加3%裂壶菌能够显著提高斑马鱼感染创伤弧菌后的存活率，相对保护率为42.8%；Real-time RT-PCR检测的结果发现S组的斑马鱼肾脏中溶菌酶Lysozyme的mRNA水平极显著高于对照组（P＜0.01），而肿瘤坏死因子TNFb的mRNA水平则极显著低于对照组（P＜0.01）。
结论裂殖壶菌作为营养添加剂可以提高斑马鱼抗创伤弧菌感染的能力。

Abstract:
Objective A feeding experiment was conducted in order to study the effects of feeding Schizochytrium on the resistance of fish to bacterial infection.
Methods Six-month adult zebrafishes were fed with diety added 3% Schizochytrium(Group S) for 28 days in a laboratory culture system, and the control with only basic diety(Group C). Then Vibrio vulnificus FJ03-X2 was applied for infection, and the relative survival percent of zebrafishes were analyzed. And RNA was extracted from the kidney tissues and reverse transcribed into cDNA to detect the experison of lysozyme and tnfb.
Results The survival rate of Group S was significantly increased with immune protective rate reaching 42.8%. The mRNA level of Lysozyme in the kidney of zebrafish in Group S was significantly higher than that in the control (P＜0.01), while the mRNA level of TNFb was significantly lower than that in the control group (P＜0.01).
Conclusion As a nutrient additive, Schizochytrium could improve the ability of zebrafish to resist bacterial infection, which provided a theoretical basis for the application of Schizochytrium in fishery industry.

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