海带多糖抑制平滑肌细胞增殖及抗氧化活性研究

彭臻菲; 李泳宁; 魏碧娜; 林碧珠

doi:10.19303/j.issn.1008-0384.2019.12.014

海带多糖抑制平滑肌细胞增殖及抗氧化活性研究

Inhibitory Effects of Laminaria Japonica Polysaccharides on Proliferation and Antioxidant Activities of Vascular Smooth Muscle Cells

摘要

摘要:
目的研究海带多糖对H₂O₂诱导血管平滑肌细胞(vascular smooth muscle cells, VSMC)增殖及胞内脂质过氧化物生成量的影响，为阐明海带多糖对VSMC的作用机制奠定基础。
方法以H₂O₂为诱导剂建立体外VSMC增殖模型，通过四甲基偶氮唑盐法和细胞形态观察评价海带多糖对H₂O₂诱导VSMC生长和增殖的影响，同时以丙二醛为指标考察海带多糖对H₂O₂诱导VSMC胞内脂质过氧化物生成量的影响。
结果以50 μmol·L⁻¹ H₂O₂为诱导剂建立VSMC体外增殖模型。四甲基偶氮唑盐法测定结果表明海带多糖对H₂O₂诱导VSMC增殖具有显著抑制活性，最大增殖抑制率达到73.56%。细胞形态学观察结果表明海带多糖作用下H₂O₂诱导的VSMC生长状态发生改变且细胞数量显著减少。丙二醛检测结果表明海带多糖作用下VSMC胞内脂质过氧化物量显著减少。
结论海带多糖能够抑制H₂O₂诱导的VSMC增殖，且显著降低VSMC胞内脂质过氧化物生成量。

Abstract:
Objective Effects of Laminaria japonicapolysaccharides on the proliferation and lipid peroxide production of H₂O₂-induced vascular smooth muscle cells (VSMCs) were examined in vitro for further understanding on the metabolic mechanism.
Method Proliferation of VSMCs was artificially induced by using 50 µmol·L⁻¹ H₂O₂. Inhibitory rate on the proliferation by the L. japonica polysaccharides were determined by a methyl thiazolyl tetrazolium (MTT) assay. Morphological changes on VSMCs were observed under a microscope. Malondialdehyde (MDA) was employed as an indicator of the lipid peroxide production in VSMCs.
Result A significant inhibition effect of the polysaccharides on the H₂O₂-induced VSMC proliferation with the greatest rate of 73.56% was shown by MTT. Morphologically, the number of VSMCs significantly decreased after an exposure to the polysaccharide. The MDA analysis indicated that the lipid peroxide production by VSMCs significantly declined after the treatment with L. japonica polysaccharides.
Conclusion The L. japonica polysaccharides significantly inhibited the proliferation and lipid peroxide production in the H₂O₂-induced VSMCs.

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