Abstract:
Objective Optimum preparation and antibacterial properties of a natural preservative extracted from bamboo sawdust were studied.
Method Utilizing bamboo sawdust, a natural preservative was prepared with the ethyl acetate reflux method. Processing conditions were optimized by the response surface methodology based on the results of a single factor test. In vitro antimicrobial activity of the extract was determined.
Result The finalized process applied 300 W ultrasonic power on the mixture of solvent-to-substrate ratio at 20 ∶ 1 to extract at 89℃ for 2 h. The product yield reached 4.77%. The extracted preservative displayed peak antimicrobial effects on Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Aspergillus niger with the average inhibition diameters of 9.33 mm at a concentration of 0.025 00 g·mL−1, 15.67 mm at a concentration of 0.012 5 0g·mL−1, 10.00 mm at a concentration of 0.050 00 g·mL−1, and 6.67 mm at a concentration of 0.100 00 g·mL−1, respectively.
Conclusion The natural preservative prepared by the newly developed extraction method showed strong in vitro antimicrobial effect, and the optimized process could be upscaled to fully utilize the bamboo sawdust.
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