响应面法优化鸡小肠黏膜唾液酸提取工艺

胡玥明; 孙桂菊; 姚宏亮

doi:10.19303/j.issn.1008-0384.2020.06.013

响应面法优化鸡小肠黏膜唾液酸提取工艺

Response Surface Optimization of Sialic Acid Extraction from Chicken Small-intestine Mucosa

摘要

摘要:
目的优化鸡小肠黏膜唾液酸提取工艺，为肉鸡加工副产物开发再利用提供新思路。
方法以鸡小肠黏膜为原料，以唾液酸提取量为指标，先采用单因素试验，在几种提取唾液酸常用酸中筛选最佳酸，进一步确定提取酸浓度、提取时间、液料比等3个因素影响唾液酸提取量的优化范围。在单因素试验基础上，采用Box-Behnken试验设计方法，进行3因素3水平响应面优化设计，共进行17组处理，每组处理3次重复，考察柠檬酸浓度、提取时间、液料比等3个因素对唾液酸提取量的影响，从而确定鸡小肠黏膜唾液酸提取的最佳工艺参数。
结果鸡小肠黏膜唾液酸提取最佳工艺参数为：柠檬酸浓度0.1 mol·L⁻¹，提取时间4 h，液料比32.20 ∶ 1（mL·g⁻¹）。考虑实际操作的简便，确定唾液酸提取的最佳工艺参数为：柠檬酸浓度0.1 mol·L⁻¹，提取时间4 h，液料比30 ∶ 1（mL·g⁻¹）。在此条件下，得到的唾液酸提取量为（1 010.39±21.87）μg·g⁻¹，与预测值1 025.69 μg·g⁻¹无明显差异。
结论通过响应面法优化了唾液酸提取工艺条件，提高了鸡小肠黏膜唾液酸提取量，为鸡小肠废弃物开发再利用提供新的思路。

Abstract:
Objective A process of extracting sialic acid from chicken small-intestine mucosa was optimized for the utilization of a broiler processing byproduct.
Method A single factor experiment was conducted to select appropriate acid for the extraction. Then, a 3-factors Box-Behnken test was designed to optimize the acid concentration, time, and liquid-to-material ratio in extracting sialic acid from chicken intestinal mucosa.
Result Seventeen experiments were performed in triplicate to arrive at the optimized process that utilized citric acid at a concentration of 0.1 mol·L⁻¹ in a liquid-to-material ratio of 32.20 ∶ 1 (mL·g⁻¹), which was simplified to 30 ∶ 1 (mL·g⁻¹), to extract for 4 h. The resulting extract contained sialic acid (1 010.39±21.87) μg·g⁻¹, which was close to the targeted goal of 1 025.69 μg·g⁻¹.
Conclusion The optimized sialic acid extraction from chicken small-intestine mucosa provided a new venue for byproduct utilization and waste reduction in poultry processing.

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