Abstract:
Objective To screen and isolate a cellulase-producing microbe for efficient, rapid and natural composting cattle waste.
Method At 3 cow manure composting plants in the Three Gorges Reservoir area in Chongqing, samples for microbial analysis were collected. The agar dilution method was applied to isolate bacillus strains from the compost specimens. Cellulase activity of the strains was determined for the screening by the hydrolytic circle method and the CMC enzyme saccharification method. Selected strains were morphologically examined and further identified by physiological, biochemical, and molecular biological methods. Based on 16S rRNA sequence, phylogenetic tree of the final selection was constructed using the molecular biological software MEGA6.0 for confirmation.
Result Out of 57 isolates from the compost specimens, 10 strains showed varying degrees of cellulase activity. Among them, X10 had the highest activity at 31.9 U·mL−1 and was identified on the 16S rRNA phylogenetic tree to be Bacillus amyloliquefaciens.
Conclusion X10 was a species of B. amyloliquefaciens that was highly productive in secreting cellulase to enhance the degradation of cow manure in compost.
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