• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

武夷名丛茶树种质资源茶多糖抗氧化活性分析

Antioxidant Activity of Polysaccharides in Wuyi Mingcong Tea Germplasms

    摘要
  • 摘要:
      目的  探明武夷山茶区特色武夷名丛茶树种质资源茶多糖的组成特征和抗氧化活性，为武夷名丛茶树种质资源的开发利用和乌龙茶新品种选育提供科学依据。
      方法  以31份武夷名丛茶树种质资源为试材，相同方法提取茶多糖，测定茶多糖基本组分和体外抗氧化活性，采用聚类热图分析和隶属函数法进行分类和综合评价。
      结果  31份武夷名丛茶树种质资源茶多糖的提取率为1.73%～3.39%；茶多糖组分中，中性糖含量为25.75%～67.21%，糖醛酸含量为11.10%～22.57%，蛋白质含量为2.24%～7.42%，茶多酚含量为3.81%～9.68%；茶多糖对DPPH自由基清除率为25.47%～84.30%，平均值为55.61%，对羟基自由基的清除率为34.98%～78.38%，平均值为54.20%；茶多糖基本组成和自由基清除率7个指标的变异系数在16.74%～23.71%，遗传多样性指数在1.93～2.18。聚类热图分析表明，糖醛酸、蛋白质和茶多酚含量与DPPH·和·OH自由基清除率相关性较强，而提取率和中性糖含量与DPPH·和·OH自由基清除率相关性较弱；31份武夷名丛茶树种质资源可分为3类。经隶属函数法综合评价，平均隶属函数值排名前5位的武夷名丛种质为九龙兰、玉蟾、玉笪、白鸡冠和红鸡冠。
      结论  31份武夷名丛茶树种质资源茶多糖基本组分和体外抗氧化活性存在较大的差异性和多样性，茶多糖组分中糖醛酸、蛋白质和茶多酚对其抗氧化活性贡献较大，九龙兰、玉蟾、玉笪、白鸡冠和红鸡冠 5份武夷名丛茶多糖抗氧化活性较强，可作为武夷岩茶保健育种的亲本材料。

     

    Abstract:
      Objective  Composition and antioxidant activity of polysaccharides （TPS） in Wuyi Mingcong tea germplasms from plantations at Mt. Wuyi were determined for better utilization of the existing resource and new variety breeding.
      Method  Samples of 31 Wuyi Mingcong tea germplasms were collected for polysaccharides extraction. The extraction rate and contents of neutral sugar, uronic acid, protein and tea polyphenol were determined. Methods of D-deoxyribose-iron system and DPPH assay were adopted to evaluate the scavenging activities on DPPH· and hydroxyl （·OH） free radicals. A clustered heatmap and subordinate function method were employed to characterize and classify the various TPS.
      Result  The TPS extraction rates on the tea germplasm samples ranged from 1.73% to 3.39%. The extracts contained 25.75-67.21% neutral sugars, 11.10-22.57% uronic acid, 2.24-7.42% protein, 3.81-9.68% polyphenols. The average scavenging activity of TPS on DPPH· was 55.61%, ranging from 25.47% to 84.30%, and that on ·OH 54.20%, ranging from 34.98% to 78.38%. The coefficients of variation on 7 TPS traits ranged from 16.74% to 23.71% and on genetic diversity indices from 1.93 to 2.18. The hierarchical cluster and heatmap analyses showed that the contents of uronic acid, protein, and polyphenols significantly correlated with the free radical scavenging activities of the TPS extracts, but not between the extraction rate or neutral sugar and the antioxidant activity. The 31 germplasms could be divided into 3 classes. The top 5 germplasms on the average subordinate function value were Camellia sinensis （L.） O. Kuntze cv. Jiulonglan, cv. Yuchan, cv. Yudan, cv. Baijiguan, and cv. Hongjiguan.
      Conclusion  The composition and antioxidant activity of TPS in the 31 Wuyi Mingcong teas varied significantly. The uronic acid, protein, and polyphenols in TPS exerted significant effect on its antioxidant activity. TPS in Jiulonglan, Yuchan, Yudan, Baijiguan, and Hongjiguan appeared to be higher on antioxidant activity among the germplasms. These 5 varieties could be applied for breeding new varieties to make oolong teas for the health food market.

     

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