• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

体外产气法评价发酵桑叶对湖羊瘤胃发酵参数的影响

Utilization of Fermented Mulberry Leaves to Enhance Ruminant Digestion of Hu Sheep

    摘要
  • 摘要:
      目的   研究发酵桑叶在湖羊瘤胃中的产气效果及降解率，为发酵桑叶的推广应用提供理论基础。
      方法   选用苜蓿和羊草作为试验对照，采集3头健康成年湖羊瘤胃液，利用体外产气法评估发酵桑叶在瘤胃中48 h内的产气发酵参数。
      结果   发酵桑叶48 h体外产气总量为187.25 mL·g−1，与苜蓿体外产气总量差异不显著（P＞0.05），但极显著高于羊草（P＜0.01）；发酵桑叶总可挥发性脂肪酸浓度为46.78 mmol·L−1，极显著高于羊草组（P＜0.01）；其中发酵桑叶的乙酸、丙酸、丁酸、戊酸、异丁酸和异戊酸浓度分别为29.76、10.18、4.12、1.54、0.48和0.70 mmol·L−1，除丙酸外，其他酸浓度均显著高于羊草（P＜0.01）；其中丁酸和戊酸浓度显著高于苜蓿（P＜0.01）。发酵桑叶、苜蓿和羊草发酵液中氨态氮（NH3-N）含量分别为1.03、1.75和0.71 mmol·mL−1，组间差异极显著（P＜0.01）；发酵桑叶的乙酸丙酸比值为2.92，极显著高于苜蓿和羊草（P＜0.01）；发酵桑叶的干物质降解率为46.76%，极显著低于苜蓿和羊草（P＜0.01），中性洗涤纤维降解率为70.25%，极显著高于苜蓿和羊草（P＜0.01）。
      结论   发酵桑叶能促进反刍动物瘤胃产气发酵，可以在反刍动物饲料中应用推广。

     

    Abstract:
      Objective   An in vitro gas production and nutrient degradation test was applied to determine the possibility of using fermented mulberry leaves in feed for Hu sheep.
      Method   Three healthy adult Hu sheep were fed each on alfalfa, Leymus chinensis or fermented mulberry leaves for comparison in this study. Ruminant liquids from the sheep were extracted and designated as ARL (from the sheep fed on alfalfa), LRL (from the sheep fed on L. chinensis), and MRL (from the sheep fed on the fermented mulberry leaves) for the 48 h in vitro simulated fermentation culture test. Production of selected gases and degradation of dry matters and fibers from the test were measured in 48-hour intervals during the fermentation process.
      Result   After 48h of the in vitro fermentation, the total gas produced by MRL was 187.25 mL·g−1, which was significantly higher than LRL (P＜0.01) but not with respect to ARL (P＞0.05); the 46.78 mmol·L−1 total volatile fatty acids (TVFA) significantly higher than either LRL or ARL (P＜0.01); the acetate, propionate, butyrate, valerate, isobutyrate and isovalerate, with the exception of propionate, concentrations of 29.76, 10.18, 4.12, 1.54, 0.48, and 0.70 mmol·L−1, respectively, significantly higher than LRL (P＜0.01); butyrate and valerate significantly higher than ARL (P＜0.01); and, the ratio of
    acetate to propionate is 2.92 significantly higher than LRL or ARL (P＜0.01). There were extremely significant differences on NH3-N content among the fermentation broths of MRL, ARL, and LRL at 1.03, 1.75, and 0.71 mmol·mL−1, respectively (P＜0.01). The in vitro dry matter digestibility (IVDMD) by MRL of 46.76% was extremely significantly lower than the others (P＜0.01), but the neutral detergent fiber digestibility (IVNDFD) of 70.25% significantly higher (P＜0.01).
      Conclusion   The in vitro fermentation test with the ruminant liquids employed in this study clearly demonstrated the enhancing effect of MRL in the gas production and nutrient degradation as compared to ARL or LRL. It appeared that the fermented mulberry leaves could be applied in feed to promote the ruminant digestion of Hu sheep.

     

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