• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

小麦百农矮抗58幼苗对Na2SO4胁迫响应及阈值鉴定

Response and Tolerance Threshold of Bainong Aikang-58 Wheat Seedlings toward Na2SO4 Stress

  • 摘要:
      目的  百农矮抗58是我国小麦的主栽品种之一,探讨百农矮抗58幼苗对Na2SO4胁迫响应机制,并界定其对Na2SO4胁迫耐受阈值,进而为进一步深入研究提供基础数据,也为农业生产实践提供科学依据与理论指导。
      方法  通过水培试验,设置不同浓度(0、20、40、60、80、100 mmol·L−1)Na2SO4胁迫处理,通过测定株高、鲜重、生物量、各种抗氧化酶活性、质膜透性、丙二醛(MDA)含量及渗透调节物质含量,研究Na2SO4胁迫对小麦幼苗生长与生理的影响。
      结果  随着Na2SO4浓度的升高,小麦幼苗的株高、鲜重及生物量均下降,小麦幼苗的各种抗氧化酶活性会随着Na2SO4浓度的升高而不断升高,当超过一定阈值后会下降,MDA含量和质膜透性会随着Na2SO4浓度的升高而升高,最大值分别为对照的3.20倍和4.94倍;小麦幼苗叶片的叶绿素含量随着Na2SO4浓度的升高而下降;小麦体内的可溶性糖含量随着Na2SO4浓度的升高呈先上升后下降的变化趋势,在80 mmol·L−1时可溶性糖含量最高;脯氨酸含量则随着Na2SO4浓度的升高而升高,最大值为对照的8.51倍。
      结论  根据试验结果界定小麦百农矮抗58耐Na2SO4胁迫阈值为80~100 mmol·L−1

     

    Abstract:
      Objective  Bainong Aikang-58 is one of the major wheat cultivars in China. To investigate the response mechanism of Bainon Aikang-58 seedling to Na2SO4 stress was discussed and to define its tolerance threshold to Na2SO4 stress was determined so as to provide basic data support for further research and scientific basis and and theoretical guidance for agricultural production practice.
      Method  Effects of Na2SO4 stress at 0, 20, 40, 60, 80, and 100 mmol·L−1 on the growth and physiology of the wheat seedlings were studied in a hydroponic experimentation.
      Result   As the Na2SO4 concentration in the medium increased, the plant height, fresh weight, and biomass of the seedlings decreased. The activity of each antioxidant enzyme in wheat seedlings would continuously increase with the increase of Na2SO4 concentration, and would decrease after exceeding a certain threshold. The membrane permeability and content of MDA in the synplasmic membrane increase with increasing of Na2SO4 concentration to maximize 3.2 and 4.94 times of the control. The chlorophyll content in the leaves were decreased with the increase of Na2SO4 concentration. The soluble sugar content in wheat increased first and then decreased with the increase of Na2SO4 concentration. The soluble sugar concentration was the highest at 80 mmol·L−1 Whereas, the proline content kept increasing to reach a maximum that was 8.51 times of control.
      Conclusion  The tolerance threshold of Bainong Aikang-58 wheat seedling toward Na2SO4 stress was determined to be 80−100 mmol·L−1.

     

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