鄂北岗地山药病害病原菌鉴定

杨小林; 张佑宏; 王佐乾; 常向前; 吕亮; 张舒; 杨新笋

doi:10.19303/j.issn.1008-0384.2021.01.008

鄂北岗地山药病害病原菌鉴定

Pathogens Infecting Chinese Yams Planted on Ridges in Northern Hubei

摘要

摘要:
目的开展鄂北岗地山药病害病原菌的鉴定，探索引起病害的病原菌及其分类地位，为进一步研究其发生规律及制定切实有效的防治措施提供依据。
方法采集鄂北岗地山药地上组织病害样本，利用植物病理学组织分离法和ITS-PCR技术，通过病原物培养形态、显微观察、ITS鉴定比对和致病性测定验证，进行山药病害病原菌的分离和鉴定。
结果鄂北岗地山药病害的病原真菌系链格孢属互隔交链孢霉（Alternaria alternata），刺盘孢属果生炭疽菌（Colletotrichum fructicola）和胶孢炭疽菌（Colletotrichumn gloeosporiaides），毛色二孢属假可可毛色二孢（Lasiodiplodia pseudotheobromae）。
结论以上结果揭示鄂北岗地山药有多病害发生，病原菌组成复杂。山药炭疽病及其他病害均可导致山药地上组织枯萎甚至死亡。

Abstract:
Objective Pathogens that cause serious diseases on Dioscorea batatas Decne grown on the mountain ridges in northern Hubei province were investigated to facilitate drafting effective disease prevention and control measures.
Method Specimens of aboveground parts of the diseased plants were collected for pathogen isolation. The isolates were identified by colonial morphology, microstructure, and blasting sequence of ITS as well as the pathogenicity determination with tissue separation of phytopathology and ITS-PCR.
Result The isolated pathogenic fungi included Alternaria alternate, Colletotrichum fructicola, C. gloeosporiaides, and Lasiodiplodia pseudotheobromae.
Conclusion The pathogens causing the diseases, such as anthracnose, that can lead to withering or death of Chinese yams at the region were highly diversified and complex.

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