Abstract:
Objective Characteristics and damages brought about by the soft rot on Paris polyphylla Smith var. chinensis (Franch.) Hara and the pathogen that caused the disease were studied for positive identification and field control.
Method Specimens of the diseased plant tissues were collected for isolation, purification, morphological examination, 16S rDNA gene sequencing, and pathogenicity determination of the pathogen.
Result The diseased leaves, stems, and tubers on the plants were usually found in the fields where air circulation and water drainage were poor. At onset of the disease, water-soaked necrosis and pungent odor began to appear on the infected plant parts. Then the internal tissues started to break down and liquify. The morphological, molecular biological characteristics, and pathogenicity on the isolate showed the responsible pathogen to be Pectobacterium carowvorum.
Conclusion The soft rot disease on P. polyphylla was determined to be infected by P. carowvorum under poorly ventilated surrounding and inadequately drained soil in the field where the plants were cultivated.
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