水稻褐飞虱若虫和成虫褪黑素含量测定及比较

施龙清; 解振兴; 姜照伟; 董萌; 占志雄

doi:10.19303/j.issn.1008-0384.2021.06.010

水稻褐飞虱若虫和成虫褪黑素含量测定及比较

HPLC-MS/MS Determination of Melatonin in Brown Planthopper at Growth Stages

摘要

摘要:
目的明确褪黑素在褐飞虱各发育阶段的含量情况。
方法通过高效液相色谱-串联质谱技术(HPLC-MS/MS)对1～5龄若虫和两种翅型雌、雄成虫体内褪黑素含量进行测定和比较。
结果褐飞虱若虫期褪黑素含量在1～2龄若虫时期最低，平均为378.5 pg·g⁻¹。3龄若虫褪黑素含量最高，为856.6 pg·g⁻¹。4龄若虫褪黑素含量下降，为810.7 pg·g⁻¹，与3龄无显著差异。5龄若虫褪黑素含量下降显著，为574.5 pg·g⁻¹。褐飞虱成虫褪黑素含量与性别显著相关，但受翅型影响不明显。雌、雄成虫间的褪黑素水平有极显著差异，长、短翅雄性成虫的褪黑素含量分别为978.3 pg·g⁻¹和969.6 pg·g⁻¹，而长、短翅雌性的褪黑素含量仅为76.1 pg·g⁻¹和176.8 pg·g⁻¹，甚至低于1～2龄若虫时期。
结论初步明确了褐飞虱各发育阶段体内褪黑素含量变化特点，为后续研究褐飞虱褪黑素功能及机理奠定基础。

Abstract:
Objective Melatonin contents in brown planthopper, Nilaparvata lugens (Stl), at various developmental stages were determined.
Method HPLC-MS/MS was used to measure the melatonin contents in the 1^st to the 5^th instar nymphs as well as female and male macropterous and brachypterous adults of the insect.
Result At nymph stage, N. lugens had the lowest average melatonin content of 378.5 pg·g⁻¹ in the 1^st and the 2^nd instars, the highest of 856.6 pg·g⁻¹ in the 3^rd instar, 810.7 pg·g⁻¹ in the 4^th instar, and 574.5 pg·g⁻¹ in the 5^th instar. Adult N. lugens differed significantly on melatonin contents between male and female but not between the macropterous and brachypterous wing types. The male of macropterous and brachypterous wing types contained 978.3 pg·g⁻¹ and 969.6 pg·g⁻¹ melatonin, respectively, which were extremely significantly higher than the female counterparts at 76.1 pg·g⁻¹ and 176.8 pg·g⁻¹, respectively.
Conclusion The melatonin contents in N. lugens as nymphs and adults were determined.

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