• 中文核心期刊
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一例鸡细小病毒、鸡传染性贫血病毒及鸡传染性支气管炎病毒共感染病例的检测

A Case of Co-infection by Chicken Parvovirus, Infectious Anemia Virus, and Infectious Bronchitis Virus on Broilers

    摘要
  • 摘要:
      目的  对福建省南平市某肉鸡场不明病因造成肉鸡死亡的病原进行确诊。
      方法  通过对鸡传染性支气管炎病毒（Infectious bronchitis virus，IBV）、腺病毒（Fowl adenovirus，FadV）、鸡细小病毒（Chicken parvovirus，ChPV）、新城疫病毒（Newcastle disease virus，NDV）、鸡传染性贫血病毒（Chicken infectious anemia virus，CIAV）、传染性法氏囊病毒（Infectious bursal Disease virus，IBDV）、喉气管炎病毒（Laryngotracheitis virus，LTV）、禽肾炎病毒（Avian nephritis virus，ANV）等病原进行PCR检测，并对扩增片段进行克隆测序，利用生物信息学软件对测序结果进行分析。
      结果  PCR检测结果及测序结果显示：鸡细小病毒ChPV、鸡传染性支气管炎病毒IBV、鸡传染性贫血病病毒CIAV为阳性，其余病原检测结果均为阴性。同源性分析结果显示分离株与ChPV的同源性为92.17%～100%，与IBV的同源性为76.9%～84.3%，与CIAV的同源性为91.19%～92.14%；遗传进化树分析结果显示分离到的IBV毒株及CIAV毒株均独处于一个单独的分支，分离到的ChPV毒株与广西参考株亲缘关系较近。
      结论  从南平市某鸡场检测到ChPV、IBV、CIAV等3种病原的共感染病例，分离到的IBV毒株的S1基因及CIAV毒株的VP1基因可能已经发生变异。

     

    Abstract:
      Objective   Pathogens causing broiler death by unknown etiology at a chicken farm in Nanping, Fujian were investigated.
      Methods  To establish standard references for this investigation, pathogens on chicken, such as, infectious bronchitis virus (IBV), fowl adenovirus(FadV), chicken parvovirus (ChPV), newcastle disease virus(NDV), chicken infectious anemia virus (CIAV), chicken infectious bursal virus(IBDV), laryngotracheitis virus(LTV), and avian nephritis virus (ANV), were detected by PCR and the amplified fragments cloned, sequenced, and analyzed using bioinformatics software.
      Results  The clinic specimens were found positive on ChPV, IBV, and CIAV but negative on the other viral pathogens. The homology of the isolates was 92.17%–100% with ChPV, 76.9%–84.3% with IBV, and 91.19%–92.14% with CIAV. The isolated IBV and CIAV strains were in their own unique branch, while ChPV closely related to a Guangxi strain as shown by the phylogenetic analysis.
      Conclusion  A case of simultaneous co-infection by ChPV, IBV, and CIAV at a chicken farm was confirmed. The S1 gene of the isolated IBV and the VP1 in the CIAV strain appeared to have mutated prior to the infection incidence.

     

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