• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

李果斑病病原菌生物学特性及室内毒力测定

Biological Characteristics and Lab Toxicity Test Results of Plum Fruit Spot Pathogen

  • 摘要:
      目的  明确李果斑病病原菌(Fusarium fujikuroi)的生物学特性并筛选出对李果斑病有效的防治药剂。
      方法  测定不同培养基、光照、温度、pH和碳、氮源对病原菌生长的影响,并采用菌丝生长速率法测定7种杀菌剂对该病原菌的室内毒力。
      结果  该病原菌最适生长温度25 ℃,pH为7~8,最适培养基为Czapek和PDA,最适碳源为淀粉和葡糖糖,最适氮源为蛋白胨和硝酸钠,不同光照条件对菌丝生长无显著影响;室内毒力测定结果表明7种杀菌剂对病原菌菌丝生长均有抑制效果,其中45%咪鲜胺WDG、20%吡噻菌胺SC和30%丙硫菌唑OD对该菌室内毒力较强,其EC50值分别为0.8914、0.9436、1.0220 μg·mL−1
      结论  明确了对李果斑病病原菌生长的最适温度、pH、光照及营养物质等,筛选对该病原菌有较好抑制作用的杀菌剂,为李果斑病的后续研究提供基础数据及有效防控措施的选择提供参考。

     

    Abstract:
      Objective  Biological characteristics of the pathogen that caused the plum fruit spot disease, Fusarium fujikuroi, were studied. Various agents for effective control of the disease were tested in the laboratory.
      Method  Effects of culture medium, light exposure, temperature, pH, and carbon/nitrogen sources on the growth of F. fujikuroi were determined. Toxicities of 7 fungicides to the pathogen were compared with the mycelium growth rate method in the laboratory.
      Result  F. fujikuroi grew well on Czapek or PDA medium at pH of 7-8 and 25 ℃. The preferred carbon sources were starch and glucosaccharide, and those for nitrogen, peptone and sodium nitrate for the culture. Different light exposures did not significant affect the fungal growth. The 7 fungicides displayed significant but varying inhibitory effects on the mycelial development. Among them, 45% imidazolide WDG, 20% pyrathiazolide SC, and 30% prothiazolium OD showed high toxicities with EC50 of 0.891 4, 0.9436, and 1.022 0 μg·mL−1, respectively.
      Conclusion   The effects of temperature, pH, light exposure, and nutrients on the growth of F. fujikuroi were clearly shown by this study. The relative efficacies of 7 commonly available fungicides for controlling the plum fruit spot disease were compared.

     

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