10个玉米<i>ZmbZIP</i>基因表达模式分析

贾利强; 吴洪梅; 曾科美; 龙金云; 郑飞; 赵秋芳; 陈曙

doi:10.19303/j.issn.1008-0384.2021.11.001

10个玉米ZmbZIP基因表达模式分析

Expression Patterns of Various Maize ZmbZIPs

摘要

摘要:
目的对10个玉米ZmbZIP基因应答逆境胁迫表达进行分析，以期为进一步深入研究玉米bZIP基因在玉米生长发育或者抵御各类逆境胁迫中的作用机制等提供科学数据。
方法设置200 mmol·L⁻¹的NaCl溶液、20% PEG6000 （Polyethylene Glycol 6000）、4 ℃低温和硝态氮或铵态氮缺乏胁迫试验，选取10个ZmbZIP基因，探测其应答响应表达模式。
结果进化分析结果揭示10个ZmbZIP可以细分为5个亚组，其保守区域分布在碱性结构域和亮氨酸拉链结构域等部分。组织表达分析结果表明10个ZmbZIP基因的表达模式不同，预示着在玉米生长进程中的不同功能。人为模拟盐、干旱、低温和氮缺乏胁迫等逆境，结果表明不同的胁迫处理下10个ZmbZIP基因的表达水平存在较大的差异，预示着在应对逆境胁迫途径中广泛的作用。
结论 10个ZmbZIP基因在玉米扬花期不同组织中，以及应答不同逆境胁迫条件下的表达模式不同，预示着ZmbZIP基因广泛参与玉米生长发育进程及逆境胁迫响应调控途径。

Abstract:
Objective Expression patterns of 10 ZmbZIPs in Zea mays under stresses were analyzed for further understanding of the biological functions of bZIP associated with the plant development and stress responses.
Method Expressions of the ZmbZIPs under imposed stresses of 200 mmol·L⁻¹ NaCl, 20% PEG6000, 4℃, and nitrogen deficiency were studied.
Result The evolutionary analysis divided the 10 ZmbZIP genes with a conserved domain consisting of a basic region and a leucine zipper into 5 subgroups. These genes expressed differently in different tissues indicating their diverse roles in the development of a maize plant. And they showed significantly differentiated expression patterns under the simulated salt, drought, low temperature, and nitrogen deficiency stresses reflecting their diverse and important roles played in the stress signaling pathways.
Conclusion Differentiations on the expression patterns of 10 ZmbZIPs in different tissues and under different stresses as demonstrated in this study revealed diversity in biological functions of the genes in maize.

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