• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

基于代谢组学分析马齿苋根茎叶中4种重要次生代谢产物

Metabolome Analysis on Four Important Secondary Metabolites in Roots, Stems, and Leaves of Portulaca oleracea L.

  • 摘要:
      目的  探究马齿苋重要次生代谢产物成分及其在不同部位相对含量及联系。
      方法  以马齿苋根茎叶为研究对象,利用高效液相色谱串联质谱进行代谢组学分析。
      结果  马齿苋中生物碱、类黄酮、香豆素与木脂素以及萜类所含的种类及化合物分别有3类43种、7类34种、2类16种和3类5种,热图分析表明,这4类次生代谢产物在马齿苋茎和叶中的相对含量较根部更为接近;生物碱在根部和叶部总体含量较高;类黄酮在茎部总体含量较高;萜类以及香豆素与木脂素在根茎叶部含量依次递增。
      结论  马齿苋不同部位所含生物碱、类黄酮、香豆素与木脂素以及萜类种类相同,但相同成分在不同部位含量差异较大。

     

    Abstract:
      Objective  Contents and interrelations of 4 important secondary metabolites in parts of a Portulaca oleracea L. plant were studied.
      Method  Metabolomics of P. oleracea root, stem, and leaf were analyzed using high performance liquid chromatography combined with tandem mass spectrometry (HPLC-MS/MS).
      Result  There were 3 categories of 43 alkaloids, 7 categories of 34 flavonoids, 2 categories of 16 lignans and coumarins, and 3 categories of 5 terpenoids identified in the metabolites. A heatmap analysis showed the relative contents of the 4 important secondary metabolites to be similar in the stems and leaves but different from what in the roots. The alkaloids content was higher in the roots and leaves, while the flavonoids in the stems. Whereas the contents of terpenoids, lignans, and coumarins were increasingly more abundant from the roots to the stems and the leaves.
      Conclusion  Same in kind, but significantly different in quantity, on the alkaloids, flavonoids, terpenoids, lignans, and coumarins were found in the roots, stems, and leaves of a P. oleracea plant.

     

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