• 中文核心期刊
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  • 中国科技核心期刊
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日粮中添加芦丁对湖羊瘤胃细菌菌群多样性及其组成的影响

Effects of Dietary Rutin on Rumen Microbial Community Diversity and Composition of Hu Sheep

    摘要
  • 摘要:
      目的  研究日粮中添加芦丁对湖羊瘤胃细菌菌群多样性及组成的影响。
      方法  选择3月龄左右、健康且体重相近的湖羊36只，随机分为3个处理，每个处理12只（公母各半，分栏饲养）。对照组（CON组）饲喂基础日粮，试验组R50和R100分别在基础日粮中添加50、100 mg·kg−1（湖羊体重基础）的芦丁。试验期为70 d，预试期14 d，正试期56 d。
      结果  1）3组共产生446个OTUs（Operational taxonomic units），其中共有OTU为396个，占总OTU的88.79%，所有样品共注释了14个门（Phylum）、18个纲（Class）、21个目（Order）、34个科（Family）和99个属（Genus）。2）相对于CON组，R100组的ACE指数、Shannon指数、Chao1指数显著降低（P＜0.05）。但CON和R50组中Alpha多样性均无显著差异（P＞0.05）。3）通过PCoA分析（Principal co-ordinates analysis）、NMDS分析（Non-metric multi-dimensional scaling analysis）、UPGMA聚类方法（Unweighted pair group method with arithmetic mean clustering analysis）和Anosim分析可知，R100组显著影响微生物群落结构。4）在门水平，拟杆菌门（Bacteroidetes）和厚壁菌门（Firmicutes）为两大优势菌门。相比CON组，R100组髌骨细菌门（Patescibacteria）的相对丰度显著提高，迷踪菌门（Elusimicrobia）的相对丰度显著降低（P＜0.05），但R50组Elusimicrobia的相对丰度显著高于CON组和R100组（P＜0.05）。5）在属水平，优势菌属均为普雷沃菌属_1（Prevotella_1）。在已鉴定丰度排名前30的属水平上，R100组新月形单胞菌属_1（Selenomonas_1）和琥珀酸菌属（Succiniclasticum）的相对丰度显著低于CON组（P＜0.05），但与R50组差异不显著（P＞0.05）。
      结论  添加100 mg·kg−1的芦丁能够影响湖羊瘤胃微生物多样性。

     

    Abstract:
      Objective  Effects of rutin supplementation in forage on the rumen microbial community diversity and composition of Hu sheep were studied.
      Method   Thirty-six healthy 3-month-old Hu sheep of similar body weight were randomly divided into 3 groups of 12 animals (half male and female) each for the treatments. The sheep in the control group (CON) were fed a basal diet, while those in two treatment groups on a daily diet supplemented with rutin at a rate of 50 mg (R50) or 100 mg (R100) per kg of animal body weight. The feeding test lasted for 70 d that included 14 d of pre-test and 56 d of actual test.
      Result  (1) Of the 446 OTUs collected in the sheep rumens, 396 (constituted 88.79% of total) were commonly shared by the sheep in the 3 groups that annotated into 14 phyla, 18 classes, 21 orders, 34 families, and 99 genera. (2) The ACE, Shannon, and Chao1 indices of the sheep in the R100 group were significantly lower than those of CON (P＜0.05), while the alpha diversity not significantly different between CON and R50 groups (P＞0.05). (3) The results of the principal co-ordinates analysis (PCoA), the non-metric multi-dimensional scaling analysis (NMDS), the unweighted pair group method with arithmetic mean clustering analysis (UPGMA), and the anosim analysis showed that the microbial community was significantly altered by the R100 treatment (P＜0.05). (4) Bacteroidetes and Firmicutes were two dominant phyla. R100 significantly increased the relative abundance of Patescibacteria but decreased that of Elusimicrobia (P＜0.05). On the other hand, R50 significantly raised the relative abundance of Elusimicrobia over CON or R100 (P＜0.05). (5) At genus level, the dominant microbes were Prevotella_1. The relative abundance of Selenomonas_1 and Succiniclasticum in the sheep fed with R100 were significantly lower than CON (P＜0.05) but not significantly different from R50 (P＞0.05).
      Conclusion   Addition of rutin to forage at a rate of 100 mg·kg−1 of sheep body weight significantly affected the rumen microbial diversity in Hu sheep .

     

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