Objective   The diversity of culturable bacteria and their inter-species interactions were explored by isolating and culturing sporophore site soil bacteria of R. griseocarnosa. It provides insights into the mycorrhizal helper bacteria which may promote the growth of the mycelium and the formation and differentiation of sporophore of R. griseocarnosa.

  Methods   The bacteria in the sporophore site soil of R. griseocarnosa were isolated and purified by traditional methods of separation and culture using LB and ISP₂ media, and the species of the isolated strains was preliminarily identified by 16S rRNA gene sequence analysis. Interactions between culturable bacterial genera were analyzed using the Bipartite package in R.

  Results   A total of 128 strains of bacteria were isolated from 6 sporophore site soils of R. griseocarnosa, which belonged to 34 OTUs (16 genera of 3 phyla). The three genera with the highest abundance were Bacillus , Burkholderia and Streptomyces. Genus-level-Sample network analysis showed that the distribution of culturable bacteria was randomly nested. Bacillus and Burkholderia are important groups in the community, which have more Effective Partners, Closeness and high Species Strength in the bacterial community.

  Conclusion   There are abundant bacterial resources in the sporophore site soil of R. griseocarnosa. Combining the analysis of bacterial abundance and network structure can predict the interaction between bacteria more clearly.