Abstract:
Objective In order to provide theoretical basis for the development, utilization and protection of Spathiphyllum germplasm resources, the genetic diversity and relationship of germplasm resources were discussed.
Method ISSR markers were used to analyze the genetic relationship and genetic diversity of 21 Spathiphyllum germplasm resources from 14 cultivars and 7 hybrid strains.
Result Nine clearly amplified and highly polymorphic ISSR primers were used for PCR amplification of 21 germplasm. A total of 133 loci were amplified, including 120 polymorphic loci, and the polymorphism ratio (PPB) was 90.23%. According to NTsys cluster analysis, the 14 cultivars could be divided into three groups at the similarity coefficient of 0.6692. The 7 hybrid strains could be divided into two groups at the similarity coefficient of 0.6700. Using TBtools correlation and principal component analysis, 14 cultivars were divided into two groups on the first principal coordinate, which proved the accuracy of cluster analysis results.
Conclusion ISSR molecular markers could effectively reveal the genetic diversity and the genetic relationship between cultivars; the genetic diversity of the spathiphyllum is abundant. ISSR clustering results were in good agreement with the germplasm origin and the character of spathes; principal component analysis confirmed the clustering results and showed the genetic relationship between varieties more directly.
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