生防细菌蜡样芽孢杆菌BCCY-22发酵条件优化

赵月; 杨亚楠; 李雅华; 赵洪海; 咸洪泉

doi:10.19303/j.issn.1008-0384.2022.007.015

生防细菌蜡样芽孢杆菌BCCY-22发酵条件优化

Optimizing Fermentation of Biocontrol Bacterium Bacillus cereus BCCY-22

摘要

摘要:
目的蜡样芽孢杆菌BCCY-22是有效防治多种线虫病害的生防细菌。本研究旨在探明生防菌蜡样芽孢杆菌BCCY-22的最优培养条件，为实现该菌株的高效发酵奠定基础。
方法以蜡样芽孢杆菌BCCY-22的OD₆₀₀为指标，采用单因素试验和响应面法对该菌株发酵条件进行优化。
结果高温、低初始pH和供氧不足显著影响蜡样芽孢杆菌BCCY-22的生长，最适发酵条件：发酵温度21.5 ℃、初始pH 7.3、种子液接种量3%、装瓶量23%、发酵时间36 h。
结论蜡样芽孢杆菌BCCY-22优化发酵条件后，缩短了发酵时间6 h，生物量是优化前的135.94%，研究为蜡样芽孢杆菌BCCY-22生防菌的发酵生产和开发利用提供了依据。

Abstract:
Objective Conditions for optimal fermentation of Bacillus cereus BCCY-22, which is known to be capable of effectively controlling a variety of nematode diseases, were determined.
Methods Using OD₆₀₀ of the fermentation broth as the indicator, optimal culture conditions for the process were determined with a single factor and response surface experimentation.
Results High temperature, low initial pH, and restricted oxygen supply significantly governed the bacterial growth. The finalized process was carried out at 21.5 ℃ with an initial pH at 7.3 and inoculation of the seeding broth at 3% to fill 23% in a flask for 36 h.
Conclusion The optimization shortened the fermentation time by 6 h and increased the biomass by 135.94%. The improvements materially provided the basis for industrialized utilization of B. cereus BCCY-22 as a biocontrol agent.

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