Objective  The phosphatidyl ethanolamine binding protein (PEBP) in Cannabis sativa L. (hemp) was identified and bioinformatics of the gene family analyzed.

  Method  Using the PEBP family of arabidopsis as reference, the existing genome data on C. sativa were collected by NCBI, MEME, and TBtools.

  Result  Twelve PEBPs were identified from the database. They were divided into 3 subfamilies as FT-like, TFL-like, and MET-like. These hydrophilic proteins were distributed in cytoplasm and nucleus with the characteristic motif1-motif5 of cannabis PEBP that located on 7 chromosomes. The genes contained 2 exons and more than 2 introns with a promoter containing mainly light-responsive cis-regulatory elements and various hormone cis-regulatory elements. The encoded protein composed basically of random coils.

  Result  Twelve genes in the PEBP family of C. sativa were identified with their potential molecular functions analyzed.