大黄鱼变形假单胞菌的遗传学和血清学聚类特征初步分析

许斌福; 陈秀锦; 池洪树; 徐梦婷; 陈佳; 林能锋; 龚晖

doi:10.19303/j.issn.1008-0384.2022.009.001

大黄鱼变形假单胞菌的遗传学和血清学聚类特征初步分析

A Preliminary Study on Genetic and Serological Clustering of Pathogenic Pseudomonas plecoglossicida in Larimichthys crocea

摘要

摘要:
目的内脏白点病是冬春季大黄鱼（Larimichthys crocea）最主要的细菌性疾病，变形假单胞菌（Pseudomonas plecoglossicida）是内脏白点病的主要病原。分析大黄鱼来源的变形假单胞菌病原聚类特征，为大黄鱼变形假单胞菌流行规律研究和科学防控提供参考。
方法克隆7株不同时空来源的大黄鱼变形假单胞菌16S rDNA、gyrB，测序并构建进化树；制备上述菌株的O-特异链血清（O抗原血清）与鞭毛蛋白血清（H抗原血清），进行菌体凝集试验。
结果 16S rDNA和gyrB序列分析表明，所有的变形假单胞菌聚为一簇；O抗原和H抗原凝集结果表明，7株变形假单胞菌以及变形假单胞菌标准株均会发生凝集反应，凝集效价也无明显区别，与荧光假单胞菌（Pseudomonas fluoresenes）、溶藻弧菌（Vibrio alginolyticus）和哈维氏弧菌（Vibrio harveyi）不发生凝集反应。
结论本研究所用的7株不同时空来源的变形假单胞菌亲缘关系接近，可能具有共同的起源。

Abstract:
Objective Clustering of strains of Pseudomonas plecoglossicida, a major pathogen that causes the serious bacterial visceral white spot disease on Larimichthys crocea in winter and spring, was studied for disease control and epidemic prevention.
Method The 16S rDNA and gyrB of 7 suspected P. plecoglossicida strains collected from different temporal and spatial sources were cloned and sequenced with evolutionary trees constructed. O and H antigen serums of these strains were prepared for agglutination tests.
Result The 7 pathogens could be clustered into a same branch based on their sequences. Their O and H antigen sera agglutinated with those of the standard P. plecoglossicida reference without significant differences on titers, but not with that of P. fluoresenes, Vibrio alginolyticus, or V. harveyi.
Conclusion It appeared that the 7 collected strains of P. plecoglossicida were genetically closely related and might have a common origin.

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