喷施茉莉酸甲酯对烟草叶片氧化酶活性及叶际微生物多样性的影响

吴加香; 刘涛; 谢永辉; 黄之远; 字淑慧

doi:10.19303/j.issn.1008-0384.2022.06.005

喷施茉莉酸甲酯对烟草叶片氧化酶活性及叶际微生物多样性的影响

Effect of Spraying Methyl Jasmonate on Oxidase Activity and Interleaf Microbial Diversity of Tobacco Leaves

摘要

摘要:
目的探索茉莉酸甲酯（MeJA）影响烟草抗氧化酶活性与叶际微生物多样性及其相关性。
方法设喷施0.5 mmol·L^-1茉莉酸甲酯（+MeJA）和不喷施（CK）处理，在喷施72 h后分别测定烟草叶片的超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性、总酚含量和过氧化氢（H₂O₂）积累量，烟草叶际细菌、真菌的丰富度和多样性。
结果与−MeJA相比， +MeJA显著提高了烟叶中SOD和POD的活性及H₂O₂的积累，显著降低叶际细菌和真菌的丰富度，极显著提高叶际真菌的多样性；SOD活性和H₂O₂积累量与高氏白粉菌属呈显著负相关，POD活性与青枯菌属和伯克霍尔德氏菌-卡巴拉尼亚-帕拉伯克霍尔德氏菌属呈正相关。
结论烟草喷施茉莉酸甲酯不仅能激活叶片中抗氧化酶活性，而且能改变叶际真菌优势菌属占比，烟草叶片中氧化酶活性与真菌菌属占比具有一定的相关关系。

Abstract:
Objective Effects of spraying methyl jasmonate (MeJA) on the antioxidant enzyme activity and interleaf microbial diversity of tobacco leaves were investigated.
Method Superoxide dismutase (SOD) activity, peroxidase (POD) activity, total phenolic content, hydrogen peroxide (H₂O₂) accumulation as well as abundance and diversity of interleaf bacteria and fungi in tobacco leaves were measured 72 h after spraying the plant growth regulator at 0.5 mmol·L^-1 (+MeJA) or without (CK) as control.
Result The MeJA spray significantly increased SOD and POD activities and H₂O₂ accumulation but reduced the microbial abundance and increased the fungal diversity in the tobacco leaves. The SOD activity and H₂O₂ accumulation significantly inversely correlated with Golovinomyces, whereas POD positively correlated with Ralstonia and Burkholderia-Caballeronia-Paraburkholderia.
Conclusion Tobacco leaves sprayed by MeJA not only activated the antioxidant enzyme activity but also altered the dominant fungi distribution in them, resulting in a correlation between oxidase activity and fungal genus share in tobacco leaves.

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