Abstract:
Objective The gene related to Sclerotinia-resistance in Brassica napus was cloned and studied to provide information for the development of disease-resistant rapeseed cultivars by means of molecular marker-assisted breeding.
Method Using the rapeseed plants known to be either highly resistant or highly susceptible to sclerotinia stem rot, the homologous P5CR (pyrroline-5-carboxylate reductase) on A03 and C03 chromosomes were amplified, cloned, sequenced, and expression analyzed. DNAMAN software was used to compare the sequencing results to locate the relevant SNP sites. Expressions of P5CR before and 6 h, 12 h, 24 h, and 48 h after inoculation into rapeseed plants were detected by qPCR.
Result The P5CR on C03 chromosome was 1457 bp in length with 7 SNP loci, of which, 3 might be related to the disease resistance. The gene on A03 chromosome was 1526 bp in length with 15 SNP sites, of which, two might be associated with the disease resistance. The expressions of P5CR on A03 and C03 chromosomes significantly increased 24 h after inoculation.
Conclusion Multiple loci in the P5CR of B. napus could be associated with the plant resistance to sclerotinia stem rot. The significant increase on the gene expression after inoculation suggested a close relationship between P5CR and the disease resistance. Further investigation is needed to unveil the underline mechanism.
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