• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

4份临床样品中羊口疮病毒F1L、B2L和GIF基因的进化分析

Genetic Evolution of F1L, B2L, and GIF of Orf Virus in Four Clinical Samples

    摘要
  • 摘要:
      目的  研究2021年福建省部分羊场羊口疮病毒（Orf virus, ORFV）分子流行病学情况。
      方法  对2021年采集的4份病料经PCR鉴定ORFV阳性的临床样品分别克隆其F1L、B2L和GIF基因，将获得的克隆质粒送往测序公司进行测序，将测序拼接后的序列上传至GenBank数据库中，利用生物信息学软件进行对比分析。
      结果  4份临床样品中ORFV的F1L、B2L和GIF基因核苷酸序列相似性分别为98.0%～98.8%、98.4%～99.5%和97.2%～99.6%；与国内流行毒株核苷酸序列相似性分别为97.4%～99.4%、97.4%～99.9%和95.7%～99.0%；与国外流行毒株核苷酸序列相似性分别为97.5%～98.6%、97.9%～98.9%和95.6%～98.0%；与德国D1701弱毒株核苷酸序列相似性分别为96.5%～96.8%、98.0%～98.7%和96.0%～96.4%，与NZ2参考株核苷酸序列相似性分别为97.7%～98.1%、97.5%～98.1%和95.9%～96.1%。遗传进化树结果显示4份临床样品中ORFV与吉林（NA17、SY17和CL18株）、福建SJ1株和印度MP株亲缘关系较近；与美国OV-IA82株、德国D1701弱毒株、新西兰NZ2参考株和中国疫苗株亲缘关系较远；表明当前流行毒株可能是本省毒株、国内吉林GZ18株和国外印度MP株的重组毒株。
      结论  获得4份临床样品中ORFV B2L、F1L和GIF基因序列并上传至GenBank数据库中，丰富了ORFV相关基因序列的数据库，同时阐明福建省部分羊场当前ORFV的流行特征，为福建省今后有效防控Orf提供理论依据。

     

    Abstract:
      Objective   Molecular epidemiology of the Orf virus (ORFV) in the diseased sheep and goats from farms in Fujian Province in 2021 were studied.
      Method  The ORFV genes, F1L, B2L, and GIF, from 4 clinical samples of the diseased animals were confirmed positive by PCR prior to cloning for sequence determination. The data were uploaded to GenBank for a comparative analysis using bioinformatics software.
      Result   The nucleotide sequence similarities were 98.0%–98.8% on F1L , 98.4%–99.5% on B2L, and 97.2%–99.6% on GIF; those related to the domestic epidemic strains, 97.4%–99.4% on F1L, 97.4%–99.9% on B2L, and 95.7%–99.0% on G1F; those related to the foreign epidemic strains, 97.5%–98.6% on F1L, 97.9%–98.9% on B2L, and 95.6%–98.0% on G1F; those related to the German D1701 attenuated strain, 96.5%–96.8% on F1L, 98.0%–98.7% on B2L, and 96.0%–96.4% on G1F; and those related to the NZ2 reference strain, 97.7%–98.1% on F1L, 97.5%–98.1% on B2L, and 95.9%–96.1% on G1F. The phylogenetic tree showed the ORFVs closely related to the strains from Jilin (NA17, SY17, and CL18), Fujian (SJ1), and India (MP), but remotely with OV-IA82 from the USA, D1701 attenuated strain from German, NZ2 reference strain from New Zealand, and the vaccine strain from China. It appeared that the current epidemic strain might be a recombination of Fujian, Jilin GZ18, and Indian MP strains.
      Conclusion  The sequences of B2L, F1L, and GIF from the 2021 clinical specimens were uploaded to GenBank database enriching its collection. This study characterized the ORFVs found in the sheep and goats infected by the epidemic and facilitated further studies in the disease control and prevention.

     

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